4BHY
Structure of alanine racemase from Aeromonas hydrophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.804, 134.738, 192.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.320 - 3.250 |
R-factor | 0.2599 |
Rwork | 0.256 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rcq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 3.430 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.090 | 0.680 |
Number of reflections | 21300 | |
<I/σ(I)> | 11.5 | 1.8 |
Completeness [%] | 93.3 | 95.4 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |