4BHX

Crystal structure of the SCAN domain from human paternally expressed gene 3 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	287
Detector technology	CCD
Collection date	2013-01-30
Detector	RIGAKU CCD
Spacegroup name	P 65
Unit cell lengths	83.615, 83.615, 55.233
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.820 - 1.950
R-factor	0.17412
Rwork	0.172
R-free	0.22383
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e6s
RMSD bond length	0.018
RMSD bond angle	2.017
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.800	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	0.310
Number of reflections	16090
<I/σ(I)>	38	9.7
Completeness [%]	99.7	99.8
Redundancy	28	20.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				CRYSTALLIZATION WAS OBSERVED DURING PROTEIN CONCENTRATION USING VIVASPIN 20 CONCENTRATORS (SARTORIUS STEDIM BIOTECH) IN 50 MM TRIS-HCL PH 7.5, 150 MM NACL.