4BHR
Structure of the TTHA1221 type IV pilin protein from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 36.790, 36.790, 207.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.270 - 1.700 |
| R-factor | 0.21728 |
| Rwork | 0.216 |
| R-free | 0.24079 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.503 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.760 |
| Number of reflections | 19190 | |
| <I/σ(I)> | 17.1 | 3.5 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 10.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 150 MM MOPS PH 7.0, 6.0 % V/V ETHYLENE GLYCOL, 1.8 M AMMONIUM SULFATE |
