4BHD
Methanococcus jannaschii serine hydroxymethyl-transferase, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.594, 110.158, 110.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.830 |
| R-factor | 0.24474 |
| Rwork | 0.242 |
| R-free | 0.29768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kl2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 25528 | |
| <I/σ(I)> | 13.93 | 2.53 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 5.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | NAH2PO4/K2HPO4 1.8 M, PH 5.0 |
