4BH5
LytM domain of EnvC, an activator of cell wall amidases in Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 |
| Unit cell lengths | 57.404, 57.404, 129.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.400 - 1.570 |
| R-factor | 0.17044 |
| Rwork | 0.166 |
| R-free | 0.20724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ne8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.660 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.076 | 0.493 |
| Number of reflections | 64864 | |
| <I/σ(I)> | 15.9 | 2.8 |
| Completeness [%] | 96.4 | 73 |
| Redundancy | 2.89 | 2.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 293 | 0.2 M SODIUM IODIDE, 17% PEG 3350, PH 6.9 |
