4BH0
H5 (tyTy) Influenza Virus Haemagglutinin in Complex with Human Receptor Analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.346, 228.296, 71.267 |
| Unit cell angles | 90.00, 114.01, 90.00 |
Refinement procedure
| Resolution | 56.610 - 2.360 |
| R-factor | 0.21371 |
| Rwork | 0.211 |
| R-free | 0.25839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.550 | 2.490 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 81446 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 97.1 | 92.6 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350 |
