4BGX
H5 (VN1194) Influenza Virus Haemagglutinin in Complex with Human Receptor Analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.338, 101.338, 451.102 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.180 - 2.480 |
| R-factor | 0.1948 |
| Rwork | 0.194 |
| R-free | 0.21776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.245 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.150 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.070 | 0.630 |
| Number of reflections | 32249 | |
| <I/σ(I)> | 17.2 | 3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 0.05 M MGCL2, 28-30% PEG 550 |
