4BGW
Crystal Structure of H5 (VN1194) Influenza Haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.240, 101.240, 451.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.900 - 2.480 |
| R-factor | 0.21035 |
| Rwork | 0.208 |
| R-free | 0.25832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 32162 | |
| <I/σ(I)> | 15.2 | 3.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 8.9 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 0.05 M MGCL2, 28-30% PEG 550 |
