4BG8
Apo form of a putative sugar kinase MK0840 from Methanopyrus kandleri (monoclinic space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.630, 73.280, 116.380 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 29.629 - 1.960 |
| R-factor | 0.1784 |
| Rwork | 0.176 |
| R-free | 0.20860 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.192 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 60796 | |
| <I/σ(I)> | 13.3 | 2.9 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.5 M POTASSIUM THIOCYANATE, 0.1 M SODIUM ACETATE PH 4.6 AT 293 K |
