4BG5
Lyme disease associated outer surface protein BBA65 from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.810, 69.870, 58.880 |
| Unit cell angles | 90.00, 101.45, 90.00 |
Refinement procedure
| Resolution | 48.790 - 3.400 |
| R-factor | 0.16794 |
| Rwork | 0.165 |
| R-free | 0.22927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4axz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.501 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.180 | 0.370 |
| Number of reflections | 7294 | |
| <I/σ(I)> | 4.5 | 3.1 |
| Completeness [%] | 91.6 | 92.7 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 29% PEG 2000, 0.1M MES PH 6.5, 0.3M IMIDAZOL |
