4BG4
Crystal structure of Litopenaeus vannamei arginine kinase in a ternary analog complex with arginine, ADP-Mg and NO3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.320, 67.150, 78.770 |
| Unit cell angles | 90.00, 92.12, 90.00 |
Refinement procedure
| Resolution | 14.847 - 1.601 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4am1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.800 | 14.800 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 86201 | |
| <I/σ(I)> | 20.9 | 4.9 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PROTEIN WAS CRYSTALLIZED FROM 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE AND 30 % (W/V) PEG 8000 PH 6.5 AT 289 K USING HANGING DROP METHOD. |
