4BF9
Crystal structure of E. coli dihydrouridine synthase C (DusC) (selenomethionine derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.140, 94.140, 119.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.170 - 2.600 |
| R-factor | 0.17858 |
| Rwork | 0.177 |
| R-free | 0.21447 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.431 |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.800 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.843 |
| Number of reflections | 17124 | |
| <I/σ(I)> | 12.2 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MOLECULAR DIMENSIONS MORPHEUS SCREEN CONDITION D3, pH 6.5 |
