4BF7
Emericilla nidulans endo-beta-1,4-galactanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.900, 72.370, 98.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.475 - 2.001 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.19230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fob |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.770 |
| Number of reflections | 58261 | |
| <I/σ(I)> | 9.3 | 2 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 200 MM ZN ACETATE, 100 MM IMIDAZOLE, 20%(W/V) PEG 3000, PH 8.0 |
