4BF3
ErpC, a member of the complement regulator acquiring family of surface proteins from Borrelia burgdorfei, possesses an architecture previously unseen in this protein family.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2011-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.520, 68.160, 76.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.150 - 2.370 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.23020 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.150 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.070 | 0.780 |
| Number of reflections | 13663 | |
| <I/σ(I)> | 13.7 | 2.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 27% (W/V) PEG 2000 MME, 0.1M SODIUM CACODYLATE PH 6.5 |
