4BEM

Crystal structure of the F-type ATP synthase c-ring from Acetobacterium woodii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-27
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	121.170, 121.170, 150.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.200 - 2.100
R-factor	0.1837
Rwork	0.182
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yce
RMSD bond length	0.007
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.140	1.030
Number of reflections	65395
<I/σ(I)>	18.08	1.58
Completeness [%]	99.3	98.8
Redundancy	16.6	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1