4BEJ
Nucleotide-free Dynamin 1-like protein (DNM1L, DRP1, DLP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 101.470, 80.770, 208.272 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 47.574 - 3.483 |
| R-factor | 0.2522 |
| Rwork | 0.251 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3snh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.618 |
| Data scaling software | XDS |
| Phasing software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.690 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.210 | 0.680 |
| Number of reflections | 42403 | |
| <I/σ(I)> | 10.19 | 2.41 |
| Completeness [%] | 97.3 | 84.3 |
| Redundancy | 3.94 | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12% PEG3350, 50 MM K(HCOO), pH 7.5 |
