4BEI
V. cholera biofilm scaffolding protein RbmA in complex with 18-crown- 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 136.885, 136.885, 116.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.960 - 2.600 |
| R-factor | 0.22368 |
| Rwork | 0.222 |
| R-free | 0.26087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4be5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.900 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.930 |
| Number of reflections | 75002 | |
| <I/σ(I)> | 21.6 | 2.44 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES PH 7 0.2 M CACL2 30 % (V/V) PEG 400 50 MM 18-CROWN-6 |
