4BE6
V. cholera biofilm scaffolding protein RbmA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-10-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.220, 118.220, 104.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.200 - 2.050 |
| R-factor | 0.13455 |
| Rwork | 0.133 |
| R-free | 0.16230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4be5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.447 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 50815 | |
| <I/σ(I)> | 37.8 | 5.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.21 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M BIS-TRIS, PH 6, 0.2 M NACL, 10 MM SIALIC ACID |
