4BDX
The structure of the FnI-EGF tandem domain of coagulation factor XII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 96.788, 96.788, 47.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.394 - 1.620 |
| R-factor | 0.2171 |
| Rwork | 0.216 |
| R-free | 0.24740 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 14626 | |
| <I/σ(I)> | 27.6 | 3.5 |
| Completeness [%] | 99.7 | 96 |
| Redundancy | 23.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 16-18% PEG4, 0.2 M POTASSIUM ACETATE |
