4BD8
Bax domain swapped dimer induced by BimBH3 with CHAPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 |
| Unit cell lengths | 142.947, 142.947, 86.712 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.823 - 2.220 |
| R-factor | 0.2003 |
| Rwork | 0.197 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BAX DOMAIN SWAPPED DIMER INDUCED BY OCTYLMALTOSIDE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 2.350 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.070 | 0.880 |
| Number of reflections | 32132 | |
| <I/σ(I)> | 13.55 | 1.9 |
| Completeness [%] | 98.6 | 93.5 |
| Redundancy | 93.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8 M NH4SO4, 0.1 M HEPES PH 6.5, 10 MM PRCL3 |
