4BCX
gamma 2 adaptin EAR domain crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-14 |
| Detector | RIGAKU IMAGE PLATE DETECTOR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.240, 37.660, 106.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.555 - 2.000 |
| R-factor | 0.2274 |
| Rwork | 0.225 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.560 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.140 |
| Number of reflections | 9894 | |
| <I/σ(I)> | 20.1 | 10.2 |
| Completeness [%] | 97.9 | 95.1 |
| Redundancy | 9.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES/IMIDAZOLE BUFFER PH 6.5, 0.03 M EACH OF DI-, TRI-, TETRA- AND PENTA-ETHYLENEGLYCOL, 10% (W/V) PEG 20000 AND 24% (V/V) PEG400 (USING A 2:1 RATIO OF PROTEIN TO MOTHER LIQUOR) |
