4BCL
FMO protein from Prosthecochloris aestuarii 2K at Room Temperature
Replaces: 3BCLReplaces: 1BCLReplaces: 2BCLExperimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 290 |
| Detector technology | FILM |
| Detector | KODAK FILM |
| Spacegroup name | P 63 |
| Unit cell lengths | 111.900, 111.900, 98.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.178 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.347 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | NEWREF ((LYNN TEN EYCK)) |
| Refinement software | TNT (4C) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.200 |
| High resolution limit [Å] | 1.840 |
| Rmerge | 0.116 |
| Number of reflections | 46456 |
| Completeness [%] | 73.0 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | PROTEIN CRYSTALLIZES FROM SOLUTION OF 14MG/ML OF PROTEIN IN 10MM TRIS.HCL BUFFER, PH 7.8, AND 1M NACL AND 5% (W/V) NH4 SO4 |
