4BBK
Structural and functional characterisation of the kindlin-1 pleckstrin homology domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.290, 82.780, 95.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.732 - 2.100 |
| R-factor | 0.1835 |
| Rwork | 0.182 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ys3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.011 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 10324 | |
| <I/σ(I)> | 13.7 | 3.5 |
| Completeness [%] | 95.4 | 95.2 |
| Redundancy | 7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5MG/ML - 12MG/ML IN 20MM TRIS-HCL, PH 7.5, 200MM NACL, 0.5MM TCEP |
