4BBB
THE STRUCTURE OF VACCINIA VIRUS N1 Q61Y MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.718, 109.848, 70.346 |
| Unit cell angles | 90.00, 110.85, 90.00 |
Refinement procedure
| Resolution | 42.150 - 3.090 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2I39 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BUSTER-TNT |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 3.280 |
| High resolution limit [Å] | 3.100 | 3.090 |
| Rmerge | 0.070 | 0.990 |
| Number of reflections | 18168 | |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
