4BB9
Crystal structure of glucokinase regulatory protein complexed to fructose-1-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.970, 72.300, 136.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.520 - 1.470 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.17740 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 1.530 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.050 | 0.400 |
| Number of reflections | 102068 | |
| <I/σ(I)> | 14.7 | 3.9 |
| Completeness [%] | 98.6 | 98.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12-16 MG/ML IN 25 MM HEPES PH 7.4, 50 MM KCL, 1 MM MGCL2, 2 MM DTT AND 5 MM FRUCTOSE-1-PHOSPHATE 14 % PEG 8000, 20% GLYCEROL, 0.16 M CALCIUM ACETATE AND 0.08 M CACODYLATE PH 6.5 |
