4BAP
Hen egg-white lysozyme structure in complex with the europium tris- hydroxyethylcholinetriazoledipicolinate complex at 1.21 A resolution.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.509, 77.509, 38.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.755 - 1.207 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.17830 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS (JANUARY 30 2009) |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 1.270 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 36469 | |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.1 M SODIUM ACETATE PH 4.6, 0.4-1.5 M SODIUM CHLORIDE, 0.00067 M PROTEIN, 0.012 M LANTHANIDE COMPLEX, 293 K, 3-7 DAYS |
