4BAI
Mycobacterium tuberculosis Chorismate synthase before exposure to 266 nm UV laser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION GEMINI |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | AGILENT |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 131.820, 131.820, 160.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.810 - 2.300 |
| R-factor | 0.1828 |
| Rwork | 0.182 |
| R-free | 0.20420 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 34968 | |
| <I/σ(I)> | 15.1 | 3.25 |
| Completeness [%] | 94.1 | 72.2 |
| Redundancy | 4.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 4.8 M NH4-ACETATE, 0.1 M NA-ACETATE, PH 4.5, VAPOR DIFFUSION |
