4BA9
The structural basis for the coordination of Y-family Translesion DNA Polymerases by Rev1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Collection date | 2012-01-30 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.750, 105.750, 424.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.820 - 2.730 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25010 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.080 | 2.800 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.080 | 0.740 |
| Number of reflections | 24571 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM HEPES.NAOH PH 8.0, 10 MM MGCL2, 5 MM NICL2, 7-9% (W/V) PEG 3350, 4% (V/V) 2,2,2-TRIFLUOROETHANOL. |
