4BA3
mImp_alphadIBB_A89NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.829, 90.090, 99.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.100 |
| R-factor | 0.1858 |
| Rwork | 0.185 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ial |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 1.060 |
| Number of reflections | 41996 | |
| <I/σ(I)> | 19.6 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.2 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8 M SODIUM CITRATE, 0.1 M HEPES BUFFER, PH 6.5 AND 10 MM DTT |
