4B9X
Structure of extended Tudor domain TD3 from mouse TDRD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.960, 62.960, 132.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.630 - 2.800 |
| R-factor | 0.22384 |
| Rwork | 0.223 |
| R-free | 0.24418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4B9W |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 6968 | |
| <I/σ(I)> | 14.8 | 2.75 |
| Completeness [%] | 98.8 | 93 |
| Redundancy | 5.87 | 6.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 10 % PEG 1000 AND 10 % PEG 8000 AT 20C |
