4B9S
Structure of the high fidelity DNA polymerase I with an oxidative formamidopyrimidine-dG DNA lesion outside of the pre-insertion site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.066, 93.688, 105.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.730 |
| R-factor | 0.18242 |
| Rwork | 0.181 |
| R-free | 0.20643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9m |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 0.510 |
| Number of reflections | 88292 | |
| <I/σ(I)> | 24.3 | 3.9 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
