4B9Q

Open conformation of ATP-bound Hsp70 homolog DnaK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	202.331, 77.470, 182.960
Unit cell angles	90.00, 101.71, 90.00

Refinement procedure

Resolution	59.717 - 2.400
R-factor	0.1986
Rwork	0.196
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1DKG AND 1DKX
RMSD bond length	0.009
RMSD bond angle	1.099
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.720	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.088	0.490
Number of reflections	108374
<I/σ(I)>	5.23	1.55
Completeness [%]	99.6	100
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6	291	28% PEG4000, 5% GLYCEROL, 0.1 M AMMONIUM ACETATE, 0.085 M SODIUM CITRATE PH 5.6, 5 MM ATP, 10 MM MGCL2, 291 K