4B9G
Structure of CssB subunit complemented with donor strand from CssA subunit of enterotoxigenic Escherichia coli colonization factor CS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.956, 69.225, 72.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.286 - 1.040 |
| R-factor | 0.1556 |
| Rwork | 0.155 |
| R-free | 0.16650 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.722 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.280 | 1.100 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.065 | 0.480 |
| Number of reflections | 124292 | |
| <I/σ(I)> | 12 | 2.7 |
| Completeness [%] | 99.1 | 95.4 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG4000 IN 0.2 M NA ACETATE, 0.1 M TRIS-HCL, PH 8.5 |
