4B9C

Biomass sensoring modules from putative Rsgi-like proteins of Clostridium thermocellum resemble family 3 carbohydrate-binding module of cellulosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-17
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41
Unit cell lengths	39.184, 39.184, 94.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.184 - 1.171
R-factor	0.1704
Rwork	0.170
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b97
RMSD bond length	0.008
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.190
High resolution limit [Å]	1.170	1.170
Rmerge	0.080	0.800
Number of reflections	48184
<I/σ(I)>	13.7	1.3
Completeness [%]	97.5	96.5
Redundancy	3.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		0.2 M SODIUM MALONATE PH 4.0 20 % PEG 3350