4B96

Family 3b carbohydrate-binding module from the biomass sensoring system of Clostridium clariflavum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-17
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	135.212, 135.212, 54.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.278 - 1.911
R-factor	0.1546
Rwork	0.153
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CLOSTRIDIUM THERMOCELLUM 0404 CBM3B
RMSD bond length	0.019
RMSD bond angle	1.667
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.120
Number of reflections	23184
<I/σ(I)>	11.8	1.7
Completeness [%]	98.8	98.8
Redundancy	4.68	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		2.0 M NACL, 0.1 M NA ACETATE PH 4.6