4B8Y
Ferrichrome-bound FhuD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.890, 50.140, 54.350 |
| Unit cell angles | 90.00, 106.44, 90.00 |
Refinement procedure
| Resolution | 36.137 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g9q |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.317 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.620 |
| Number of reflections | 22105 | |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG-2000, 0.01M NICL2, 0.1M TRIS-HCL PH 8.5 |
