4B8V
Cladosporium fulvum LysM effector Ecp6 in complex with a beta-1,4- linked N-acetyl-D-glucosamine tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.500, 57.500, 118.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.800 - 1.590 |
| R-factor | 0.20369 |
| Rwork | 0.203 |
| R-free | 0.22504 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.805 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.590 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 31231 | |
| <I/σ(I)> | 7.8 | 2.2 |
| Completeness [%] | 98.1 | 94.1 |
| Redundancy | 9.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | ECP6 IN 20 MM HEPES, PH 7.0, AND 50 MM NACL. CRYSTALS WERE OBTAINED BY MICRO-SEEDING, USING A RESERVOIR WITH 200 MM AMMONIUM SULPHATE, 100 MM SODIUM ACETATE, PH 4.6, AND 20-30% PEG MME 200 (W/V). |
