4B8O
rImp_alpha_SV40TAgNLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.494, 73.865, 62.064 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 46.145 - 2.084 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.620 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 35070 | |
| <I/σ(I)> | 15.1 | 3.6 |
| Completeness [%] | 97.1 | 82.8 |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M BIS-TRIS PROPANE PH 7.0, 14% PEG 3350, 0.2 M NDSB-221 (SIGMA) AND 0.2 M NAF |
