4B8J
rImp_alpha1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.252, 72.736, 62.425 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 19.841 - 2.001 |
| R-factor | 0.1496 |
| Rwork | 0.148 |
| R-free | 0.17780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ee4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 40273 | |
| <I/σ(I)> | 16.2 | 5.4 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M BIS-TRIS PROPANE PH 7.0, 15-17% PEG 3350, 0.2 M NAF, 0.2 M NDSB-221. |
