4B87
Crystal structure of human DNA cross-link repair 1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.400, 83.400, 257.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.230 - 2.160 |
| R-factor | 0.17844 |
| Rwork | 0.176 |
| R-free | 0.21834 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.230 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.090 | 1.190 |
| Number of reflections | 29805 | |
| <I/σ(I)> | 15.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 17.5% PEG 3350, 0.2M KCSN PH 8.2, 5% ETHYLENE GLYCOL |
