4B7V
Structure of wild type Pseudomonas aeruginosa FabF (KASII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293.15 |
| Detector technology | CCD |
| Collection date | 2012-05-30 |
| Detector | RIGAKU SATURN 944+ HG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.790, 65.860, 84.760 |
| Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
| Resolution | 48.850 - 1.730 |
| R-factor | 0.18205 |
| Rwork | 0.180 |
| R-free | 0.21098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gfw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.850 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 76169 | |
| <I/σ(I)> | 20.8 | 2.3 |
| Completeness [%] | 94.2 | 59.7 |
| Redundancy | 4.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M MAGNESIUM CHLORIDE, 0.1 M TRIS PH 7.0, 10% (W/V) PEG8000 |
