4B7O
THE FrpB IRON TRANSPORTER FROM NEISSERIA MENINGITIDIS (F5-1 VARIANT) APOPROTEIN FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-11 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.210, 79.100, 74.750 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 37.050 - 2.320 |
| R-factor | 0.23559 |
| Rwork | 0.233 |
| R-free | 0.28067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aiq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.050 | 0.300 |
| Number of reflections | 43114 | |
| <I/σ(I)> | 9.8 | 2 |
| Completeness [%] | 98.2 | 98.8 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20MM NA L-GLUTAMATE, 20MM D, L-ALANINE, 20MM GLYCINE, 20MM LYSINE (RACEMIC), 20MM SERINE (RACEMIC), 39.8MM IMIDAZOLE, 24% (W/V) PEGMME550, 12% (W/V) PEG 20,000 AND 61.2MM MES/NAOH (PH 6.5) |
