4B7N
H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 118.350, 118.350, 68.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.620 - 2.840 |
| R-factor | 0.18706 |
| Rwork | 0.185 |
| R-free | 0.22401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nss |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.700 | 3.000 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.160 | 0.480 |
| Number of reflections | 11923 | |
| <I/σ(I)> | 15.4 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG3350, 0.2M SODIUM FLUORIDE AND 0.1M BIS-TRIS PROPANE BUFFER (PH 6.5) |
