4B7J
H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-12-18 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 118.550, 118.550, 67.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.828 - 2.417 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.834 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.830 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.130 | 0.420 |
| Number of reflections | 19102 | |
| <I/σ(I)> | 11.4 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG3350, 0.1M BIS-TRIS PROPANE, 0.1M SODIUM ACETATE BUFFER (PH 4.6) |
