4B6M
Trypansoma brucei tubulin binding cofactor B CAP-Gly domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.660, 55.730, 80.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.790 - 1.590 |
| R-factor | 0.20286 |
| Rwork | 0.201 |
| R-free | 0.24034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1whg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.444 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.180 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.070 | 0.280 |
| Number of reflections | 20384 | |
| <I/σ(I)> | 28.2 | 9.6 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 13.9 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M POTASSIUM FORMATE, 30% PEG 3350 AND A PROTEIN SOLUTION CONSISTING OF 50 MM TRIS-HCL PH 7.5, 250 MM NABR WITH THE PROTEIN AT 7.5 MG ML-1. |
