4B6J

Crystal structure of phosphoserine phosphatase from T. onnurineus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2011-09-29
Detector	RIGAKU R-AXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	89.281, 89.281, 228.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.641 - 3.340
R-factor	0.2168
Rwork	0.214
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ap9
RMSD bond length	0.005
RMSD bond angle	0.909
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.640	3.400
High resolution limit [Å]	3.340	3.340
Rmerge	0.180	0.540
Number of reflections	14085
<I/σ(I)>	9.1	2.2
Completeness [%]	99.6	99.6
Redundancy	3.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		30% PENTAERYTHRITOL ETHOXYLATE, 50 MM BIS-TRIS PH 6.5, 50 MM AMMONIUM SULFATE