4B6I
Crystal structure Rap2b (SMA2266) from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-19 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 48.055, 56.894, 122.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.100 - 1.950 |
| R-factor | 0.17824 |
| Rwork | 0.176 |
| R-free | 0.22873 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.780 | 44.780 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.080 |
| Number of reflections | 23682 | |
| <I/σ(I)> | 11.5 | 11.5 |
| Completeness [%] | 10.0 | 100 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 1 M LICL2, 20% PEG 6K, 0.1 M CITRIC ACID PH 4.0 |
