4B66
A. fumigatus ornithine hydroxylase (SidA), reduced state bound to NADP and Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.010, 84.870, 145.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.070 - 2.900 |
| R-factor | 0.20432 |
| Rwork | 0.199 |
| R-free | 0.29856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b63 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.340 |
| Number of reflections | 10567 | |
| <I/σ(I)> | 4.5 | 2.7 |
| Completeness [%] | 96.3 | 94.4 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | HANGING DROP VAPOR DIFFUSION METHOD, WITH A RESERVOIR SOLUTION CONTAINING; 1.6 M AMMONIUM SULFATE, 0.1 M HEPES, 2% DIOXANE, PH 6.6. |
