4B60
Structure of rFnBPA(189-505) in complex with fibrinogen gamma chain C- terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.510, 59.050, 73.490 |
| Unit cell angles | 91.86, 98.05, 97.87 |
Refinement procedure
| Resolution | 58.410 - 1.830 |
| R-factor | 0.20088 |
| Rwork | 0.198 |
| R-free | 0.24593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b5z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.557 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.830 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 51268 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 93.6 | 67.4 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 2K MME 25%, 0.2 M CA AC, ISOPROPANOL 10% |
