4B5E
Crystal Structure of an amyloid-beta binding single chain antibody PS2-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.647, 59.209, 133.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.314 - 1.936 |
| R-factor | 0.1841 |
| Rwork | 0.181 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b41 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.221 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.320 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 15145 | |
| <I/σ(I)> | 15 | 3.5 |
| Completeness [%] | 98.8 | 94.3 |
| Redundancy | 6.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | 0.2 M AMMONIUM SULFATE, 0.1 M NACITRATE PH 5.2, 26-28% PEGME5,000 TEMP 18 C |
